Developmental Analysis of Spliceosomal snRNA Isoform Expression
نویسندگان
چکیده
منابع مشابه
Developmental Analysis of Spliceosomal snRNA Isoform Expression
Pre-mRNA splicing is a critical step in eukaryotic gene expression that contributes to proteomic, cellular, and developmental complexity. Small nuclear (sn)RNAs are core spliceosomal components; however, the extent to which differential expression of snRNA isoforms regulates splicing is completely unknown. This is partly due to difficulties in the accurate analysis of the spatial and temporal e...
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Human proteins 15.5K and hPrp31 are components of the major spliceosomal U4 snRNP and of the minor spliceosomal U4atac snRNP. The two proteins bind to related 5'-stem loops (5'SLs) of the U4 and U4atac snRNAs in a strictly sequential fashion. The primary binding 15.5K protein binds at K-turns that exhibit identical sequences in the two snRNAs. However, RNA sequences contacted by the secondary b...
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INVOLVEMENT OF THE SPLICEOSOMAL U4 snRNA IN HETEROCHROMATIC GENE SILENCING AT FISSION YEAST CENTROMERES Madoka Chinen, Misato Morita, Kazuhiro Fukumura and Tokio Tani* From Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan Running title: U4 snRNA is involved in centromeric silencing Address correspondence to: Tokio Tani, ...
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We have determined the crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4 snRNA. The protein interacts almost exclusively with a purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual RNA fold characterized by two tandem sheared G-A base pairs, a high degree of purine stacking, and the acco...
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Lsm proteins are ubiquitous, multifunctional proteins that are involved in the processing and/or turnover of many, if not all, RNAs in eukaryotes. They generally interact only transiently with their substrate RNAs, in keeping with their likely roles as RNA chaperones. The spliceosomal U6 snRNA is an exception, being stably associated with the Lsm2-8 complex. The U6 snRNA is generally considered...
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ژورنال
عنوان ژورنال: G3 Genes|Genomes|Genetics
سال: 2015
ISSN: 2160-1836
DOI: 10.1534/g3.114.015735